Utilization of Defatted Mango Kernel in Wheat-Based Cereals Products: Nutritional and Functional Properties

The mango kernel is a byproduct of mango processing industries and it contains appreciable quantities of edible oil and quality proteins. In the present research, wheat flour was partially replaced with defatted mango kernel flour up to 30% and blends were further utilized for the preparation of biscuits. The nutritional and functional properties of flour blends were determined in the first phase. The second phase dealt with product analysis and sensorial appraisal from the trained taste panel. The results indicated that the addition of mango kernel flour improved the mineral and fiber content of flour blends. The addition of mango kernel flour affected emulsifying and foaming properties of flour blends negatively, while water and oil absorption improved positively from 60.76 ± 2.39% to 86.29 ± 2.51% and 81.81 ± 2.94% to 123.87 ± 5.39%, respectively. The results might be due to lower bulk density of blends. The cookies prepared from flour blends showed improved color tonality and textural characteristics. Sensorial appraisal from a trained taste panel was awarded to cookies containing 15% and 20% defatted mango kernel flour, however, the rating was slightly less than the control. The results were conclusive that defatted mango kernel can be used at 15% and 20% with significant consumer acceptability. Future aspects of the present research include the extraction of principal ingredients, e.g., starch and proteins, for value addition.     

Effect of eCG dose on ovarian haemodynamics,hormonal profiles and prolificacy rate when oestrus was induced during low-breeding season in Beetal goats

The objectives of the experiment were to determine the effect of two doses of equine chorionic gonadotropin (eCG) in a standard synchronization protocol based on a short-term progesterone (P₄) priming on ovarian structures and haemodynamics, concentrations of steroid hormones and prolificacy rate when oestrus was induced during low-breeding season (LBS) in Beetal dairy goats. We hypothesized that inclusion of eCG in a short-term P₄ priming-based synchronization protocol would increase the blood perfusion to ovarian structures leading to enhance oestrous and ovulatory responses and prolificacy rate in goats. Forty-two multiparous acyclic goats were blocked by body condition and, within block, assigned randomly to receive saline as control (CON), low eCG (L-eCG; 300 IU) or high eCG (H-eCG; 600 IU) dose. Initially, a controlled internal drug release (CIDR) device was placed in the anterior vagina on d −8, followed by removal of CIDR on d −3, concurrent with the administration of PGF_2α and eCG according to their respective treatments. Goats were monitored for oestrous response. B-mode and Doppler ultrasonography was performed with 12-h interval, starting from day −3 until natural breeding (day 0), and then on days 5, 10, 15 and 20 post-breeding to monitor follicular and luteal dynamics and blood flow, respectively. Blood was sampled at 0, 12, 24, 36 and 60 h after CIDR removal to quantify plasma concentrations of estradiol-17β (E₂), whereas plasma concentrations of P₄ were assayed at days 5, 10, 15 and 20 after breeding. Pregnancy and prolificacy rates were determined at day 30 and 150 after breeding, respectively. Data were analysed with mixed-effects models, and orthogonal contrasts were used to evaluate the effect of treatment [Con vs. (½ L-eCG + ½ H-eCG)] and dose of eCG (L-eCG vs. H-eCG). Data are presented in sequence as CON, L-eCG, H-eCG (LSM ± SEM). The oestrous intensity score (152.9 vs. 182.7 vs. 186.5 ± 15.1; p = .02) was greater in eCG-treated goats as compared to CON. Administration of eCG reduced the intervals to standing oestrus (66.2 vs. 41.8 vs. 48.9 h ± 5.5; p = .05), breeding (70.2 vs. 44.4 vs. 45.4 h ± 4.5; p = .03) and ovulation (84.5 vs. 61.2 vs. 63.4 h ± 6.2; p = .05) compared with CON goats. The mean growth rate of pre-ovulatory follicle was greater (1.11 vs. 1.49 vs. 1.45 mm ± 0.08; p = .01) in eCG-treated goats resulting in an increased diameter of pre-ovulatory follicle (6.27 vs. 7.20 vs. 7.31 mm ± 0.07; p < .01) and corpora lutea (6.75 vs. 8.26 vs. 8.07 mm ± 0.42; p = .04) than CON. The mean follicular blood flow did not differ among treatments; however, the mean luteal blood flow was greater in L-eCG-treated goats (0.81 vs. 1.61 vs. 1.07 cm² ± 0.12; p = .001). The mean concentrations of E₂ (4.03 vs. 5.21 vs. 4.78 pg/ml ± 0.42; p = .04) and P₄ (4.85 vs. 6.39 vs. 6.22 ng/ml ± 0.34; p = .04) were greater in eCG-treated goats. The twinning rate did not differ between treatments; nevertheless, prolificacy rate was greater (p = .04) in L-eCG-treated goats. Collectively, our data suggest that the administration of eCG improves the induction of oestrous and ovarian dynamics. Administration of L-eCG enhances prolificacy rate, therefore, a low dose of eCG might be practically beneficial to improve reproduction during LBS in acyclic Beetal dairy goats.     

Canola yield and quality enhanced with sulphur fertilization

Canola cultivars positively responded to sulphur fertilization in term of seed yield and oil quality. Oscar cultivar increased seed yield 53% as compared to control plots. Sulphur @ 45 kg ha^–1 increased seed yield, biological yield, and quality of rapeseed. Number of days to flowering (76), number of pods plant–1 (372), number of seeds pod–1 (24), plant height (173 cm), biological yield (15547 kg ha^–1), seed yield (2209 kg ha^–1), harvest index (19%), glucosinolate (μmol g–1) content (31.03 μmol g–1) and oil content (45.81%) was enhanced significantly with sulphur treated @ 45 kg ha^–1 applied plots as compared to delayed flowering (78 days), shortest plant (151 cm), pods formation (298 pods), seed pod–1 (21), biological yield (11090 kg ha^–1), seed yield (1436 kg ha^–1), and oil content (42.62%) in control plots. Among cultivars “Oscar” ranked first in different growth stages and attain more plant height (164 cm), and examined substantial number of pods plant–1 (359), seeds pod–1 (24), seed yield (2005 kg ha^–1), biological yield (14298 kg ha^–1), harvest index 17%, and oil content (46.29%) as compared to other sowed cultivars. On the basis of the result it is recommended that cultivar “Oscar” treated with sulphur @ 45 kg ha^–1 should be applied for higher yield and quality of rapeseed under the agro-climatic condition of swat valley.     

Alleviation of arsenic toxicity by phosphate is associated with its regulation of detoxification,defense, and transport gene expression in barley

Arsenic(As) contamination in soils has posed a severe threat to safe crop production. The previous studies showed the antagonism between phosphorus(P) and As in plant growth and As uptake, while the mechanisms of alleviating As toxicity by P is not completely clear. Due to the limiting P condition, it is imperative to understand how low P addition can be used to suppress arsenate As(V) uptake and the subsequent mechanisms involved. Thus in this study we investigated the effect of P addition on As uptake, anti-oxidative enzyme activity, and anti-oxidant content, and the relative expression of transport, defense, and detoxification genes using two barley genotypes differing in As toxicity tolerance. P addition significantly reduced As concentration in plant tissues, and caused the great changes in activities of catalase and superoxide dismutase, glutathione content, and the relative expression of examined genes when the plants of the two barley genotypes were exposed to 100 μmol L~(–1) As, with ZDB160(As-tolerant) being much more affected than ZDB475(As-sensitive). The current results show that P addition can alleviate As toxicity by regulating the expression of As transport, defense, and detoxification genes to a greater extent in As tolerance of barley, suggesting the possibility of controlling As uptake and toxicity by applying low amount of P fertilizers in the As-contaminated soils.     

Plant Therapy: a Nonpharmacological and Noninvasive Treatment Approach Medically Beneficial to the Wellbeing of Hospital Patients

A green atmosphere and interaction with nature (plants and flowers) can considerably improve the human senses and sanity by decreasing the stress associated with anxiety, depression, neurotic impairment, and other psychologic symptoms. The current paper reports a therapeutic horticultural study’s findings regarding the effect of indoor foliage plants and flower arrangements on health prospects of surgical patients. Two surgical wards, ward A (with foliage plants and arranged flowers) and ward B (without foliage plants and flowers), were selected for the study. A total of 270 patients were randomly assigned to either ward to give 135 patients in each. Patients admitted to ward A had significantly more optimistic and promising health improvements than those in ward B. Provision of foliage plants and flower arrangements to the patients in ward A resulted in shorter postoperative stays, lower intake of analgesics, lower pain intensity, less stress fatigue, and improved vital signs (blood pressure, heart rate, respiration rate, body temperature) as compared to patients in ward B. Moreover, analyses showed that patients in ward A had a stronger psychologic frame of mind regarding their recovery from surgery and had more positive emotions and feelings regarding their stay in the hospital. They also felt that the ward atmosphere was more calming, pleasant, and satisfactory. Additionally, small group discussions and focal interviews with ward doctors and nurses confirmed the findings of the questionnaire that foliage plants & flower arrangements create a cherished environment in the ward, reduce patients’ stress, improve medical and psychologic status of patients, and also conveye positive messages of the hospital. These findings confirm the therapeutic value of horticultural activities, particularly in surgical patients in a stressed hospital setting.     

Identification and partial characterization of potential probiotic lactic acid bacteria in freshwater Labeo rohita and Cirrhinus mrigala

Carps are the most diversified freshwater fish belonging to family Cyprinidae. Numerous probiotic and pathogenic lactic acid bacteria (LAB) have been characterized from carps. However, the diversity of these ecologically important bacteria is entirely unknown in freshwater fish of Pakistan. The present study aimed to characterize and identify the lactic acid bacteria from two carps viz. Laboe rohita and Cirrhinus mrigala and determine their antagonistic activity. Seventeen bacterial isolates were purified from the gastrointestinal tract and gills of these fish and characterized morphologically. Initially, seven isolates were screened as LAB using agar supplemented with CaCO₃. Subsequently, only two isolates CILB2 and RIL10 were selected as LAB after high‐performance liquid chromatography analysis for lactic acid production. Isolates CILB2 and RIL10 were genetically identified as Enterococcus faecalis and Weissella sp., respectively after 16S rRNA gene sequence analysis. Both strains exhibited significant antagonistic activity against common fish pathogens Streptococcus agalactiae, Staphylococcus aureus and Escherichia coli. Enterococcus faecalis CILB2 and Weissella sp. RIL10 were also found negative for haemolysis and gelatinase activities and were sensitive to ampicillin, amoxicillin, doxycycline, erythromycin, chloramphenicol and co‐trimoxazole antibiotics. The identified LAB strains may further be investigated for their potential probiotic application in fish feed and food preservation techniques.     

The circulation of unique reassortment strains of infectious bursal disease virus in Pakistan

Infectious bursal disease(IBD), caused by IBD virus(IBDV), is one of the most devastating and immunosuppressive diseases of the poultry and has been a constraint on the sustainable poultry production around the globe including Pakistan. While the disease is threatening the poultry industry, the nature of predominant strains of IBDV in Pakistan remained ill-defined. In this study, an epidemiology survey was conducted in the main chicken-farming regions of Pakistan. The batch of Pakistan IBDVs genes simultaneously covering both VP1 and VP2 were amplified, sequenced, and analyzed. The unique segmentreassortant IBDVs(vv-A/Uniq-B), carrying segment A from vvIBDV and segment B from one unique ancestor, were identified as one important type of circulating strains in Pakistan. The data also discovered the characteristic molecular features of Pakistan IBDVs, which will contribute to scientific vaccine selection and effective prevention of the disease.     

In situ immobilization of Cr and its availability to maize plants in tannery waste–contaminated soil: effects of biochar feedstock and pyrolysis temperature

Journal of Soils and Sediments - Tannery waste–contaminated soil has a high amount of several toxic chemicals and heavy metals including chromium (Cr), which makes it unsuitable for...     

Enhancing phosphorus availability,soil organic carbon,maize productivity and farm profitability through biochar and organic–inorganic fertilizers in an irrigated maize agroecosystem under semi‐arid climate

Biochar amendments offer promising potential to improve soil fertility, soil organic carbon (SOC) and crop yields; however, a limited research has explored these benefits of biochar in the arid and semi‐arid regions. This two‐year field study investigated the effects of Acacia tree biomass‐derived biochar, applied at 0 and 10 t ha^?1 rates with farmyard manure (FYM) or poultry manure (PM) and mineral phosphorus (P) fertilizer combinations (100 kg P ha^‐1), on maize (Zea mays L.) productivity, P use efficiency (PUE) and farm profitability. The application of biochar with organic–inorganic P fertilizers significantly increased soil P and SOC contents than the sole organic or inorganic P fertilizers. Addition of biochar and PM as 100% P source resulted in the highest soil P (104% increase over control) and SOC contents (203% higher than control). However, maize productivity and PUE were significantly higher under balanced P fertilizer (50% organic + 50% mineral fertilizer) with biochar and the increase was 110%, 94% and 170% than 100%‐FYM, 100%‐PM and 100% mineral fertilizer, respectively. Maize productivity and yield correlated significantly positively with soil P and SOC contents These positive effects were possibly due to the ability of biochar to improve soil properties, P availability from organic–inorganic fertilizers and SOC which resulted in higher PUE and maize productivity. Despite the significant positive relationship of PUE with net economic returns, biochar incorporation with PM and mineral fertilizer combination was economically profitable, whereas FYM along biochar was not profitable due to short duration of the field experiments.